Resource Guide: Fluorescent In Situ Hybridization (FISH)

Fluorescence in situ hybridization (FISH) is a cytogenetic technique which enables “mapping” of the genetic material of cells

First described in 1969, FISH is one of the oldest cytogenetics methods and was used as early as 1993 to determine aneuploidy for pre-implantation diagnostics. Commonly used to label DNA providing information on the location, length, and number of copies of specific genes or chromosome portions, it can be applied to all types of RNA, and is foremost utilized to detect copy numbers and location of mRNA to visualize cellular transcription activity.

• The difference between FISH and ISH
• Sample types that can be used in FISH
• Methods for generating FISH probes

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